Target Audience
Scientific Community, such as clinicians, technicians, students and researchers interested in advancing flow cytometry expertise for diagnostics and translational research.
Description
Deepen your understanding of flow cytometry with this workshop that covers key suggestions and pitfalls to avoid for polychromatic, spectral, and imaging cytometry.
This workshop can be an asset for those who are just starting to plan flow cytometry experiments, but also for more experienced participants. It will cover various topics to take into account when planning flow cytometry experiments, as well as some practical tips to ensure their success. There will also be an opportunity for clinicians and researchers to get together to implement and share knowledge and good practices in Flow Cytometry.
Successful flow cytometry analysis requires a single-cell suspension, careful management of pre-analytical and analytical variables in sample preparation, and strategies to troubleshoot technical challenges and improve data quality for research and clinical applications.
Learning Objectives
-Understanding the Fundamentals of Flow Cytometry Workflow
-Overview of Polychromatic, Spectral, and Imaging Flow Cytometry instruments
-Experimental Planning
-Sample Preparation tips & tricks
-Tissue Sample Processing in Research and Diagnosis
-Panel Design
-Controls and Standardization
-Troubleshooting and Optimization
-Bridging Research and Clinical Applications
