Target Audience
Flow cytometry specialists
Description
Participants will gain a pragmatic understanding of normal and leukemic myeloid differentiation patterns (based on more conventional and new informative markers), equipping them to identify aberrant cells and flow cytometry patterns indicative of distinct AML diagnostic entities. This skill is critical for the monitoring of measurable residual disease (MRD) using flow cytometry.
Learning Objectives
1) Identification of major differentiation trajectories in normal bone marrow (BM) and peripheral blood (PB) CD34+ hematopoietic progenitor and precursor cells (HPC).
2) Highlight the contribution of lineage infidelity markers and other leukemia associated phenotypes in the diagnosis of distinct AML entities for integration in MRD panels. Relevance of using NGF databases.
3) Outline an alternative strategy to characterize CD34+ HPC in BM and PB along AML treatment, independent of baseline diagnostic data.
